Tryp-N™ Sequencing Grade Kit
Tryp-N™ Sequencing Grade Kit with digestion solution
Product Details
Patented Tryp-N™ is a high-performance protease engineered to accelerate protein digestion and enhance sensitivity in LC-MS analyses. Perfect for both routine and challenging samples, it works at higher temperatures (55 – 65 °C) to complete digestion in minutes to not many hours. This breaks down tough proteins while deactivating unwanted enzymes. Its activity can be precisely controlled with EDTA: limited proteolysis is fully reproducible.
Tryp-N™ cuts N-terminally to lysine and arginine residues, generating spectra dominated by b-ions. With up to a 35.4x increase in sensitivity over trypsin, you get cleaner, more accurate data; it’s ideal for use in Multiple Reaction Monitoring (MRM) experiments. It produces predominant b-ion fragments, simplifying analysis for deeper protein coverage and post-translational modification studies.
Product Contents
| K001‑TRYPN‑0010uG | K001‑TRYPN‑0020uG | Composition | |
|---|---|---|---|
| Tryp‑N™ Sequencing Grade Enzyme | 10 µg | 20 µg | 50 mM Trimethylammonium Acetate, 2 mM CaCl₂, 100 µM MnCl₂ in LC‑MS grade Water |
| Tryp-N™ Digestion Solution | 250 µL | 500 µL | 100 mM Trimethylammonium Acetate pH 7.4 @ 55 °C, 4 mM CaCl₂ and 200 µM MnCl₂ |
Note: This product is shipped on dry ice and is non-returnable.
