S-Trap™ Midi Spin Columns

S-Trap™ Midi Spin Columns for proteomics samples (≤ 1 mg)

Product Details

The patented ProtiFi™ S-Trap™ technology combines robust Sodium Dodecyl Sulfate (SDS) solubilization of biological samples and enhanced contaminant removal for proteomic analysis by mass spectrometry.

S-Trap™ Midi Spin Columns allow for the solubilization, reduction, alkylation, and digestion of ≤ 1 mg of total protein cleaned and ready for peptide analysis.

Product Contents

	C002‑MIDIX‑0010PK	C002‑MIDIX‑0020PK	C002‑MIDIX‑0040PK
S‑Trap™ Midi Spin Column	10	20	40

Note: This product is non-returnable.

Protocol

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“…I was planning to spend all day analyzing a viral TMT PAWS dataset in R, but I uploaded it to simplifi https://simplifi.protifi.com and it did the comparisons I needed in minutes. with GO and pathways too! Now I need to find something else to do…”

Brett Phinney, X (@UCDProteomics)

“My intern helped to do a quick & dirty comparison of few shotgun #proteomics sample preparation methods to figure out which one we should carry to next stage of automation… I think the answer is quite clear 🙂 @ProtifiLlc“

Yiming Chen, X (@ChenYM)

“1,245 proteins (min 2 peptides) from 500 clinical cells! Using @ProtifiLlc s-trap”

Yishai Levin, X (@YishaiLevin)

“The S-Trap has changed sample processing in our lab dramatically. Using SDS for sample preparation is no longer hampered by the need to precipitate and resolubilize sample pellets prior to digestion. The fast digestion times enhance our phosphopeptide recovery and enable a wide range of sample quantities (from 10 ug to 10 mg) to be processed efficiently.”

John Neveu, Harvard University, Cambridge, MA

“The S-Trap is honestly AMAZING! We just finished processing approximately 200,000 cells for a complex analysis and compared it to FASP on our QE Plus. We found a significant increase in overall quantifiable peptides. The results gave us higher confidence in our analysis and we plan on purchasing at least 3 more boxes right away! S-Traps are simply fantastic!”

Stanley M. Stevens, Jr., PhD, Stevens Lab