S-Trap™ Mini Kit
S-Trap™ Mini Spin Column Kit for proteomics with solutions (100 – 300 µg)
Product Details
The patented ProtiFi™ S-Trap™ technology combines robust Sodium Dodecyl Sulfate (SDS) solubilization of biological samples and enhanced contaminant removal for proteomic analysis by mass spectrometry. The S-Trap™ Mini Kit provides all the solutions for the solubilization, reduction, alkylation, and digestion of 10 or 80 samples containing 100 μg – 300 μg of total protein. The kit fully removes a broad range of contaminants incompatible with downstream proteomics analysis, such as detergents, salts, buffers, stabilizers, excipients, and others, while yielding high peptide recovery.
Product Contents
| K002‑MINIX‑0010KT | K002‑MINIX‑0080KT | |
|---|---|---|
| S-Trap™ Mini Spin Columns | 10 | 80 |
| S-Trap™ Solution 1 | 2x Lysis Solution (1 x 500 µL) | 2x Lysis Solution (2 x 1.5 mL) |
| S-Trap™ Solution 2 | Reductant (1 x 80.0 µL) | Reductant (1 x 100 µL) |
| S-Trap™ Solution 3 | Alkylator (1 x 80.0 µL) | Alkylator (1 x 200 µL) |
| S-Trap™ Solution 4* | Acidifier (1 x 100 µL) | Acidifier (1 x 550 µL) |
| S-Trap™ Solution 5 | Binding/Wash Solution (2 x 900 µL) (8.0 mL bottles) | Binding/Wash Solution (4 x 3.0 mL) (30.0 mL bottles) |
| S-Trap™ Solution 6 | Digestion Solution (2 x 1.5 mL) | Digestion Solution (1 x 30.0 mL) |
| S-Trap™ Solution 7 | Elution Solution (1 x 1.0 mL) | Elution Solution (1 x 9.0 mL) |
Note: This product is non-returnable. All provided solutions, including Binding/Wash Solution with MeOH added, can be stored for 1 month at 4 ºC or up to 1 year at – 20 ºC. After thawing, mix thoroughly to ensure any precipitants are redissolved.
*Acidifier must be stored tightly capped; it will degrade upon atmospheric exposure.
