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S-Trap™ MAM Kit: Standardized Sample Preparation for Multi-Attribute Method Analysis

The S-Trap™ MAM Kit brings
S-Trap™ sample processing to multi-attribute method (MAM) analysis of protein biotherapeutics. It is designed to eliminate method development and standardize processing across products, matrices and formulations. From bioreactor harvests to purified drug products, the kit provides an automation-compatible 96-well workflow that removes all detergents, salts and other contaminants, to allow digestion with your selected protease. The workflow is designed to minimize artifactual preparation-induced modifications like deamidation and oxidation. It delivers critical quality attributes (CQAs) that are truly reflective of the original sample while also providing improved detection of low-abundance host cell proteins (HCPs). For deeper characterization, complementary Tryp-N™ digestion ladders can increase sequence coverage and improve localization of post-translational modifications (PTMs). The kit provides scalable, standardized sample preparation for biotherapeutic characterization, process monitoring and quality-control workflows.

The S-Trap™ MAM Kit brings S-Trap™ sample processing to multi-attribute method (MAM) analysis of protein biotherapeutics. It is designed to eliminate method development and standardize processing across products, matrices and formulations. From bioreactor harvests to purified drug products, the kit provides an automation-compatible 96-well workflow that removes all detergents, salts and other contaminants, to allow digestion with your selected protease. The workflow is designed to minimize artifactual preparation-induced modifications like deamidation and oxidation. It delivers critical quality attributes (CQAs) that are truly reflective of the original sample while also providing improved detection of low-abundance host cell proteins (HCPs). For deeper characterization, complementary Tryp-N™ digestion ladders can increase sequence coverage and improve localization of post-translational modifications (PTMs). The kit provides scalable, standardized sample preparation for biotherapeutic characterization, process monitoring and quality-control workflows.

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S-Trap™ MAM Protocols

“…I was planning to spend all day analyzing a viral TMT PAWS dataset in R, but I uploaded it to simplifi https://simplifi.protifi.com and it did the comparisons I needed in minutes. with GO and pathways too! Now I need to find something else to do…”

Brett Phinney, X (@UCDProteomics)

“My intern helped to do a quick & dirty comparison of few shotgun #proteomics sample preparation methods to figure out which one we should carry to next stage of automation… I think the answer is quite clear 🙂 @ProtifiLlc“

Yiming Chen, X (@ChenYM)

“1,245 proteins (min 2 peptides) from 500 clinical cells! Using @ProtifiLlc s-trap”

Yishai Levin, X (@YishaiLevin)

“The S-Trap has changed sample processing in our lab dramatically. Using SDS for sample preparation is no longer hampered by the need to precipitate and resolubilize sample pellets prior to digestion. The fast digestion times enhance our phosphopeptide recovery and enable a wide range of sample quantities (from 10 ug to 10 mg) to be processed efficiently.”

John Neveu, Harvard University, Cambridge, MA

“The S-Trap is honestly AMAZING! We just finished processing approximately 200,000 cells for a complex analysis and compared it to FASP on our QE Plus. We found a significant increase in overall quantifiable peptides. The results gave us higher confidence in our analysis and we plan on purchasing at least 3 more boxes right away! S-Traps are simply fantastic!”

Stanley M. Stevens, Jr., PhD, Stevens Lab

About Multi-Attribute Method (MAM)

About Our Technology

S-Trap™ MAM Kit: Standardized Sample Preparation for Multi-Attribute Method Analysis

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