Reproducible homogenization is obligatory to omics analyses with published reports revealing that sample processing causes 75% of the total error in proteomics with the vast majority, 72%, coming from sample extraction. PIXUL™ plus ProtiFi™ S-Trap™ and Si-Trap sample processing solve this problem. PIXUL™ is the first and only truly parallel multi-sample megasonicator. It is fast, simple, and inexpensive to operate. PIXUL delivers extremely consistent 2 MHz megasonication of 1 – 96 samples. This is a significantly higher frequency than other sonication systems which operate in the kilohertz range and delivers correspondingly shorter wavelengths to samples: 0.74 mm rather than 3 mm to >3 cm. PIXUL's tight waves provide the highest level of uniformity in homogenization and significantly reduce the presence of hot and cold spots. In addition, the cavitation events which do the work of sonic treatment occur at this high frequency to afford rapid sample processing. PIXUL is compact, 45 cm x 59 cm x 34 cm, and requires no special training nor consumables. PIXUL™ homogenization with S-Trap lysis buffer is the most efficient protein extraction system available and works for samples of all types including automated processing of FFPE samples. PIXUL stands for PIXelated ULtrasound is is unrivaled in its power and convenience. With PIXUL™, sample preparation is no longer an “art form”, but instead robust workflow that gives repeatable results day in and day out.
High-throughput sonication has been limited by the cost of consumables including expensive proprietary tubes, plates, and reagents. In contrast, PIXUL uses low cost, round-bottom plates that are directly compatible with cell culture and fixation, as well as the S-Trap and Si-Trap sample processing systems. This eliminates inconvenient sample transfer steps and reduces sample loss.